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Image Search Results
Journal: Cells
Article Title: PARP1, DIDO3, and DHX9 Proteins Mutually Interact in Mouse Fibroblasts, with Effects on DNA Replication Dynamics, Senescence, and Oncogenic Transformation
doi: 10.3390/cells13020159
Figure Lengend Snippet: Interaction of DIDO3 with PARP1 and DHX9. ( A ) Western blots of samples from transfected NIH-3T3 cells expressing GFP, as a negative control, or HA-tagged-full-length DIDO3, -DIDO2 or -DIDO3-CT, untreated (−) or treated with Olaparib (+). The cells were lysed (left) and immunoprecipitated with anti-HA antibody (right); the blots were probed for PARP1, DHX9, and HA-tagged proteins. Olaparib treatment increased the co-precipitation of both proteins only in the case of full-length DIDO3 (red box). ( B ) Western blots of WT and DIDO3 mutant (dE16) cell lysates and their precipitates, previously untreated (−) or treated with Olaparib (+). Precipitation was performed with magnetic beads covalently linked to Af1521 Macrodomain, which specifically binds to PARylated proteins. The blots were probed with anti-DHX9 antibody (upper), anti-PARP1 antibody (middle) and anti-PAR antibody (lower panel).
Article Snippet:
Techniques: Western Blot, Transfection, Expressing, Negative Control, Immunoprecipitation, Mutagenesis, Magnetic Beads