gst resin Search Results


proteinlso gst resin  (TransGen biotech co)
90
TransGen biotech co proteinlso gst resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Proteinlso Gst Resin, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteinlso gst resin/product/TransGen biotech co
Average 90 stars, based on 1 article reviews
proteinlso gst resin - by Bioz Stars, 2026-06
90/100 stars
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gst resin  (GenScript corporation)
90
GenScript corporation gst resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Resin, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst resin/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gst resin - by Bioz Stars, 2026-06
90/100 stars
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af1521 macrodomain affinity resin set  (Tulip Biolabs)
90
Tulip Biolabs af1521 macrodomain affinity resin set
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Af1521 Macrodomain Affinity Resin Set, supplied by Tulip Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af1521 macrodomain affinity resin set/product/Tulip Biolabs
Average 90 stars, based on 1 article reviews
af1521 macrodomain affinity resin set - by Bioz Stars, 2026-06
90/100 stars
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gst-resin genscript  (GenScript corporation)
90
GenScript corporation gst-resin genscript
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Resin Genscript, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-resin genscript/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gst-resin genscript - by Bioz Stars, 2026-06
90/100 stars
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high-affinity gst resin  (GenScript corporation)
90
GenScript corporation high-affinity gst resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
High Affinity Gst Resin, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-affinity gst resin/product/GenScript corporation
Average 90 stars, based on 1 article reviews
high-affinity gst resin - by Bioz Stars, 2026-06
90/100 stars
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beyogold gst-tag purification resin p2253  (GenScript corporation)
90
GenScript corporation beyogold gst-tag purification resin p2253
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Beyogold Gst Tag Purification Resin P2253, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/beyogold gst-tag purification resin p2253/product/GenScript corporation
Average 90 stars, based on 1 article reviews
beyogold gst-tag purification resin p2253 - by Bioz Stars, 2026-06
90/100 stars
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gst-resin  (Becton Dickinson)
90
Becton Dickinson gst-resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Resin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-resin/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
gst-resin - by Bioz Stars, 2026-06
90/100 stars
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gst resin  (Promega)
90
Promega gst resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Resin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst resin/product/Promega
Average 90 stars, based on 1 article reviews
gst resin - by Bioz Stars, 2026-06
90/100 stars
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gst-binding resin magnegsttm glutathione particles  (Promega)
90
Promega gst-binding resin magnegsttm glutathione particles
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Binding Resin Magnegsttm Glutathione Particles, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-binding resin magnegsttm glutathione particles/product/Promega
Average 90 stars, based on 1 article reviews
gst-binding resin magnegsttm glutathione particles - by Bioz Stars, 2026-06
90/100 stars
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gst bind resin  (Merck KGaA)
90
Merck KGaA gst bind resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Bind Resin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst bind resin/product/Merck KGaA
Average 90 stars, based on 1 article reviews
gst bind resin - by Bioz Stars, 2026-06
90/100 stars
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gst-sefinose resin  (Promega)
90
Promega gst-sefinose resin
a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. <t>GST/GST-TaPIF4</t> were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with <t>Proteinlso</t> GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.
Gst Sefinose Resin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-sefinose resin/product/Promega
Average 90 stars, based on 1 article reviews
gst-sefinose resin - by Bioz Stars, 2026-06
90/100 stars
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gst or nickel resin chromatography  (GenScript corporation)
90
GenScript corporation gst or nickel resin chromatography
Porcine epidemic diarrhea virus N protein interacted with Sp1 and inhibited its transcriptional activity. (A and B) Interaction of PEDV N protein with Sp1 or Sp3 in the HEK293T cells cotransfected with recombinant plasmids pCMV-N-Flag and pCMV-Sp1-HA or -Sp3-HA. The total cell lysates were immunoprecipitated with HA antibody, followed by immunoblotting with PEDV N monoclonal antibody. Total cell lysates immunoprecipitated with normal mouse IgG were used as controls. (C) Interaction of PEDV N protein with Sp1 in the PEDV-infected IPEC-J2 cells. The cell lysates immunoprecipitated by Sp1 antibody were immunoblotted with PEDV N antibody. Uninfected cell lysates were used as a control. (D) PEDV N protein affected Sp1 binding to the putative HDAC1 promoter (bp −1 to −500), as shown by ChIP assay. (E) PEDV N protein inhibited Sp1 transcriptional activity on the HDAC promoter in the HEK293T cells transfected with different combinations of pCMV-Sp1 and/or pCMV-N together with pGL3-Enhancer-PHDAC1 and pRL-TK. (F and G) Expression and purification of <t>GST-Sp1</t> and His-N protein detected by SDS-PAGE: lane 1, <t>total</t> <t>proteins</t> after induction with IPTG; lane 2, recombinant proteins purified by affinity chromatography with a GST-binding resin or a His-binding resin. (H) PEDV N protein inhibited Sp1 binding to the HDAC promoter, as shown by gel shift assay. Migration of the target DNA was detected by 0.8% agarose gel electrophoresis.
Gst Or Nickel Resin Chromatography, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst or nickel resin chromatography/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gst or nickel resin chromatography - by Bioz Stars, 2026-06
90/100 stars
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a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.

Journal: Nature Communications

Article Title: Natural variation of STKc_GSK3 kinase TaSG-D1 contributes to heat stress tolerance in Indian dwarf wheat

doi: 10.1038/s41467-024-46419-0

Figure Lengend Snippet: a TaSG-D1/TaSG-D1 E286K interact with the C-terminus of TaPIF4 in a Y2H assay. 35 mM 3-amino-1,2,4-triazole (3-AT) was used to suppress self-activation in the TaSG-D1 E286K and TaPIF4 interaction assay. SD-L/W stands for media lacking Leucine and Tryptophan. SD-L/W/H/A stands for media lacking Leucine, Tryptophan, Histidine and Adenine. b TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in N. benthamiana leaves in an LCI assay. The experiment was performed five times. c TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vitro in a pull-down assay. GST/GST-TaPIF4 were incubated with His-TaSG-D1/His-TaSG-D1 E286K . Protein mixture was immunoprecipitated with Proteinlso GST Resin and detected with anti-His (upper panel) and anti-GST (lower panel) antibodies. The arrow in lower panel (~75 KDa) indicates GST-TaPIF4. “**” in the upper panel indicates non-specific band, the arrow in the upper panel indicates non-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K (~48 KDa), “*” in the upper panel indicates self-phosphorylated His-TaSG-D1/His-TaSG-D1 E286K . Three independent experiments were performed. d TaSG-D1/TaSG-D1 E286K interact with TaPIF4 in vivo in a Co-IP assay. 35 S: TaSG-D1-GFP/35 S: TaSG-D1 E286K -GFP/35 S: GFP and 35 S: TaPIF4-Myc were co-expressed in N. benthamiana leaves. Protein extract was immunoprecipitated with anti-GFP magnetic beads and detected using anti-GFP (lower panel) and anti-Myc (upper panel) antibodies. Three independent experiments were performed. Source data are provided as a file.

Article Snippet: For pull-down assays of interaction, 5 µg GST or GST-TaPIF4 coupled with Proteinlso GST Resin (TransGen Biotech, DP201-01) were incubated with 5 µg His-TaSG-D1 (pET-28a)/5 µg His-TaSG-D1 E286K (pET-28a) at 4 °C for 2 h with gentle mixing.

Techniques: Y2H Assay, Activation Assay, In Vitro, Pull Down Assay, Incubation, Immunoprecipitation, In Vivo, Co-Immunoprecipitation Assay, Magnetic Beads

Porcine epidemic diarrhea virus N protein interacted with Sp1 and inhibited its transcriptional activity. (A and B) Interaction of PEDV N protein with Sp1 or Sp3 in the HEK293T cells cotransfected with recombinant plasmids pCMV-N-Flag and pCMV-Sp1-HA or -Sp3-HA. The total cell lysates were immunoprecipitated with HA antibody, followed by immunoblotting with PEDV N monoclonal antibody. Total cell lysates immunoprecipitated with normal mouse IgG were used as controls. (C) Interaction of PEDV N protein with Sp1 in the PEDV-infected IPEC-J2 cells. The cell lysates immunoprecipitated by Sp1 antibody were immunoblotted with PEDV N antibody. Uninfected cell lysates were used as a control. (D) PEDV N protein affected Sp1 binding to the putative HDAC1 promoter (bp −1 to −500), as shown by ChIP assay. (E) PEDV N protein inhibited Sp1 transcriptional activity on the HDAC promoter in the HEK293T cells transfected with different combinations of pCMV-Sp1 and/or pCMV-N together with pGL3-Enhancer-PHDAC1 and pRL-TK. (F and G) Expression and purification of GST-Sp1 and His-N protein detected by SDS-PAGE: lane 1, total proteins after induction with IPTG; lane 2, recombinant proteins purified by affinity chromatography with a GST-binding resin or a His-binding resin. (H) PEDV N protein inhibited Sp1 binding to the HDAC promoter, as shown by gel shift assay. Migration of the target DNA was detected by 0.8% agarose gel electrophoresis.

Journal: Journal of Virology

Article Title: Porcine Epidemic Diarrhea Virus Inhibits HDAC1 Expression To Facilitate Its Replication via Binding of Its Nucleocapsid Protein to Host Transcription Factor Sp1

doi: 10.1128/JVI.00853-21

Figure Lengend Snippet: Porcine epidemic diarrhea virus N protein interacted with Sp1 and inhibited its transcriptional activity. (A and B) Interaction of PEDV N protein with Sp1 or Sp3 in the HEK293T cells cotransfected with recombinant plasmids pCMV-N-Flag and pCMV-Sp1-HA or -Sp3-HA. The total cell lysates were immunoprecipitated with HA antibody, followed by immunoblotting with PEDV N monoclonal antibody. Total cell lysates immunoprecipitated with normal mouse IgG were used as controls. (C) Interaction of PEDV N protein with Sp1 in the PEDV-infected IPEC-J2 cells. The cell lysates immunoprecipitated by Sp1 antibody were immunoblotted with PEDV N antibody. Uninfected cell lysates were used as a control. (D) PEDV N protein affected Sp1 binding to the putative HDAC1 promoter (bp −1 to −500), as shown by ChIP assay. (E) PEDV N protein inhibited Sp1 transcriptional activity on the HDAC promoter in the HEK293T cells transfected with different combinations of pCMV-Sp1 and/or pCMV-N together with pGL3-Enhancer-PHDAC1 and pRL-TK. (F and G) Expression and purification of GST-Sp1 and His-N protein detected by SDS-PAGE: lane 1, total proteins after induction with IPTG; lane 2, recombinant proteins purified by affinity chromatography with a GST-binding resin or a His-binding resin. (H) PEDV N protein inhibited Sp1 binding to the HDAC promoter, as shown by gel shift assay. Migration of the target DNA was detected by 0.8% agarose gel electrophoresis.

Article Snippet: Both proteins were expressed in soluble fractions and purified by GST or nickel resin chromatography (GenScript, Nanjing, China) by following the manufacturer’s instructions.

Techniques: Virus, Activity Assay, Recombinant, Immunoprecipitation, Western Blot, Infection, Control, Binding Assay, Transfection, Expressing, Purification, SDS Page, Affinity Chromatography, Gel Shift, Migration, Agarose Gel Electrophoresis